The hours of operation for Mayo Clinic in Florida can vary depending on the specific department or service. Generally, the main clinic operates during regular business hours, typically from Monday to Friday, between 8:00 AM and 5:00 PM. Some departments may also offer extended hours or weekend availability for certain services like urgent care or specialty clinics. It is important for patients to check the specific hours for the department they are interested in, as these can change based on staffing and patient demand. For the most accurate and current information regarding hours of operation, it is advisable to visit the official website or contact the clinic directly.