If you've travelled on multiple airlines, the airline that takes you to your destination is always responsible for retrieving your bag, even if you departed on a different airline.
The issue in GetHuman-volkerkr's own words
Fligt Nr. LUK**J*I booked my flight **.*.**** for * people, long before the new baggage rule was published. Before we check in, we want to be certain what happens with our ** kg each hand luggage which then we were allowed to hand to the agent before Boarding. If we have to deposit now at the check in counter, is this still free of charge in view of the early booking?
GetHuman-volkerkr did not yet indicate what Ryanair should do to make this right.
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